Swine acute diarrhea syndrome coronavirus (SADS-CoV) was reported in China in 2017 and is a causative agent of porcine enteric disease. Together, GeneTM is a potential alternative VTM with comparable RT-qPCR performance and stability to those of standard media. The delta Ct values of the SARS-CoV-2 genes in each transport medium were maintained for 14 days at cold (4☌) or room (25☌) temperatures, suggesting viral samples were stably preserved in the transport media for 14 days. The comparison of Ct values from the positive samples showed a high correlation ( = 0.95-0.96) between GeneTM and eNAT™, indicating a comparable viral detection capacity. The cycle threshold (Ct) values of three SARS-CoV-2 targets (the S, RdRP, and N genes) were analyzed using RT-qPCR testing. To evaluate the guanidine thiocyanate-based Gene Transport Medium (GeneTM) as an alternative VTM, we prepared 39 SARS-CoV-2-positive and 7 SARS-CoV-2-negative samples in GeneTM, eNAT™, and phosphate-buffered saline (PBS). Guanidine thiocyanate-based media reportedly inactivate SARS-CoV-2 and are compatible with quantitative reverse transcription polymerase chain reaction (RT-qPCR) assays, but the compatibility and the viral detection capacity have not been fully validated. Currently, the viral transport medium (VTM) required for testing is in short supply due to a sharp increase in the laboratory tests performed, and alternative VTMs are needed to alleviate the shortage. An accurate and rapid molecular diagnosis is essential to determine the exact number of confirmed cases. Severe acute respiratory syndrome coronavirus 2 SARS-CoV-2), which causes coronavirus disease 2019 (COVID-19), is still rapidly spreading as of March 2022.
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